Free Technical Info
RheoSense
RheoSense has developed a viscosity test protocol for its m VROC viscometer that achieves viscosity measurement using only 20µl (microliter) of sample fluid. According to RheoSense, this micro sample measurement is the lowest in the industry.
Oxford Gene Technology (OGT)
A new report from OGT explores the need for biomarkers as indicators of disease, and discusses the vast potential of autoantibodies as markers of early disease development. Autoantibodies are produced by the host immune system and as such are often detectable during the early stages of disease onset. In addition, they are stable, abundant, highly specific and easily purified from serum, making them ideal candidates for use in disease detection. The issues surrounding the use of autoantibodies as biomarkers are discussed in detail in the whitepaper, including their relative advantages, selection of the most appropriate discovery technology and choosing between strategies for in-house or outsourced discovery.
Genevac
A report that details how Genevac's miVac Duo sample concentrator has been used by the Australian Sports Drug Testing Lab to develop a new sample preparation method to help curb sports doping is now available. Using a miVac Duo sample concentrator the lab was able to significantly improve their sample preparation method for synthetic insulin in serum, reducing sample preparation times from several days to just 2 hours. In addition, better and more consistent recoveries were obtained than using the traditional nitrogen blow down evaporation method.
Exeter Analytical
Developed in conjunction with several leading environmental testing labs, a protocol for direct determination of carbon (C), hydrogen (H) and nitrogen (N) composition in air and water total filter samples using an Exeter Analytical Model 440 CHN Elemental Analyzer is described in Exeter Analytical application note 210.
Exeter Analytical
A new report discusses how CHN elemental analyzer design affects operational accuracy and precision, ease of use and handling of demanding sample types. The report examines the consequences of different commercial CHN elemental analyzer designs when the systems are run in real lab environments.
Data shows the impact of horizontal versus vertical combustion systems. In a horizontal furnace arrangement the sample is introduced into the combustion tube on a quartz ladle, which critically enables the removal of all sample residues after combustion. In a vertical furnace arrangement the samples are combusted on top of previously combusted samples. This difference is a major factor contributing to the advantages of a static system with a horizontal furnace over both the dynamic and hybrid designs. The build-up of sample residue in the combustion zone of vertical furnace systems is shown to considerably increase the potential for poor analytical data.
According to Exeter, the most important criteria for CHN analysis in the majority of analytical laboratories is for optimal accuracy and precision across a wide range of sample types. With constant pressure to increase laboratory productivity users do not want to set up analyzers with different operational parameters for every different sample type they come across. Test data from an independent multi-laboratory study is included in the white paper demonstrating not only the accuracy and precision routinely achievable, but also how the inherently longer term stability with a horizontal furnace design markedly decreases time lost due to the need for recalibrations and sample re-runs.
Considerable variance in instrument performance can also be seen with more demanding samples. The advantages of a horizontal furnace design CHN elemental analyzer is illustrated with applications including time-dependent combustible samples and volatile liquids.
More: sales@eai1.com or info@exeteranalytical.co.uk (Europe)
TTP LabTech
Designed specifically for users who perform protein crystallography and available for free, TTP LabTech's LabCrystal magazine delivers in-depth information about the applications of mosquito Crystal and mosquito LCP, alongside views from users across Europe, the UK and America. LabCrystal provides methodological and technical advice, and highlights recent advances in the field.
Thermo Fisher Scientific
Thermo Fisher Scientific's now offers interactive online troubleshooting for analysts to get technical advice to help resolve common problems with liquid chromatography (LC), gas chromatography (GC) and solid phase extraction (SPE) protocols. The interactive "Troubleshoot an Issue"" tool is available at the Thermo Scientific Chromatography Resource Center.
Phenomenex
Guard Finder is a new, interactive online tool designed to help users quickly select column protection products for over 41,000 HPLC, UHPLC and preparative HPLC columns. Users can search by brand, part number, technique or column phase. The tool matches input with the recommended Phenomenex column protection.
AMSBIO
A new technical article from AMSBIO can enable neuroscience researchers to learn more about a new method to control and direct differentiation of physiologically relevant neural cell types using stem cell qualified reagents. The new method describes how to differentiate adult neural stem cells and pluripotent ES cells using 3D extracellular matrices and reagents from AMSBIO.
More: www.amsbio.com/news/whitepaper/Stem_Cells_in_Neuroscience_Research.pdf
Phenomenex
This next generation calculator generates customized, optimized Kinetex methods for HPLC or UHPLC systems. With user input, which is entered online, the calculator quickly produces an optimized method based on the user's running conditions. The enhanced calculator incorporates all six Kinetex phases, including the new phenyl-hexyl chemistry, and calculates a new gradient program to account for differences in column dimensions and adjustments in running conditions. Users may also now adjust parameters such as flow rate, column length, I.D. and backpressure and quickly see a revised, optimized method. The Kinetex Calculator helps users apply advanced core-shell technology to current methods to produce better results, increase sample throughput and decrease solvent consumption.
Oxford Gene Technology
OGT's new interactive website includes white papers, application notes and a discussion forum in addition to information about products and services. The navigation structure allows users to rapidly select relevant research areas, including clinical genetics, genomic services and diagnostic biomarkers.
More: www.ogt.co.uk
Integrated DNA Technologies (IDT)
A new, free-to-download, guide from IDT provides protocols and an extensive troubleshooting section for mutagenesis applications. Everything from primer design, reaction setup, ligation and transformation efficiency, to specific reaction components and PCR parameters, are covered. The guide provides a useful reference tool for performing site-directed or random mutagenesis. The guide also describes IDT's range of Ultramer Oligonucleotides.
Thermo Fisher Scientific
This collection of videos contains updates and technical explanations of topical issues in HPLC, GC and SPE. The full portfolio of presentations, hosted on the Chromatography Videos Channel in the Thermo Scientific Chromatography Resource Center (CRC). The CRC also offers 6000 technical application notes detailing chromatographic and protocol specifications, a comprehensive library of background literature, an HPLC method transfer calculator and a GC column selector tool.
Thermo Fisher Scientific
This newly organized website provides application and method development information to help guide any HPLC and GC separation and sample preparation challenge. It includes a library of 6,000 application notes, technical bibliography, video animations and demonstrations, HPLC method development calculator, and GC column selector tool.
Thermo Fisher Scientific
A new poster and several technical notes demonstrate how a new range of Thermo Scientific KingFisher nucleic acid purification kits can be used to simply and rapidly harvest DNA and RNA to produce an excellent yield at a high level of purity and demonstrate how to extract biological factors from blood DNA, plant DNA, cell and tissue DNA, total RNA, and viral NA.
SP Scientific
This new technical brief presents protocols and calculations to determine the force required for a freeze dryer to securely seat the stopper in the vial of choice, while eliminating the use of excess pressure that may crush vials or warp shelf assemblies.
More: www.spscientific.com
Spinnovation
A detailed cast study by Spinnovation examines the utility of NMR profiling methodology to monitor and optimize stem cell culture media for the production of biologics and cells for therapeutic purposes.
Integrated DNA Technologies
Providing an overview of in vitro mutagenesis, along with in-depth protocols and troubleshooting advice, this new downloadable guide describes traditional mutagenesis approaches, including site directed mutagenesis by polymerase chain reaction (PCR), primer extension, inverse PCR and cassette-based methods. The guide also covers random mutagenesis, a technique that is often used to optimize protein expression or function by creating unpredictable alterations.
Integrated DNA Technologies
New webinar videos in IDT's online Tech Vault now include "Technical Tips for qPCR Assay Design and Setup" and "Using Free Online Tools for Oligonucleotide Analysis and Design". In addition, the Tech Vault includes a Frequently Asked Questions area and a Reading Room with educational resources, technical reports, user guides, protocols, an archive of IDT's Decoded Newsletter and more.
More: www.idtdna.com
AMSBIO
AMSBIO has added a new suite of video presentations and a bibliography of downloadable technical papers that demonstrate the utility of alvetex, a synthetic scaffold that enables routine 3D cell culture.
alvetex is a highly porous polystyrene scaffold engineered into a 200µm thick membrane that provides a 3D space into which cells can invade, proliferate and grow. The vertical axis of the third dimension enables additional layers of cells to form complex 3D interactions with adjacent cells simulating the structure of a tissue. According to AMSBIO, alvetex is easier to use, has an infinite shelf life and is less expensive than existing 3D culture products.
AMSBIO
A new AMSBIO web page brings together technical articles written by the company and its customers, offering information about stem cell biology, apoptosis, cell invasion and migration, cell signaling, DNA damage, glycobiology, cell based assay technology and 3D cell culture techniques.
TTP LabTech
Entitled "mosquito Crystal for the Rapid Screening and Optimization of Crystallization Conditions of the Bacterial Periplasmic Binding Protein, HbpA and the Elucidation of its Role as a Glutathione Import Protein," this new application note describes how the mosquito Crystal was used to dispense 100 nL crystallization droplets (50 nL protein sample and 50 nL crystallisation condition). This allowed rapid and efficient screening of almost 2000 crystallization conditions, ultimately resulting in the successful crystallization and identification of a novel GSH import protein.
More: http://www.ttplabtech.com/products/mosquito/bibliography.html
Thermo Fisher Scientific
Highlighting the importance of sample preparation within the analytical laboratory, the HyperSep SPE column range provides a resource for the purification of a wide range of analytes. Containing information on the full list of HyperSep columns available, this 144-page bench-top resource provides technical specifications and method information to assist separations scientists in selecting the appropriate phase for their needs. Conveniently divided into pharmaceutical, environmental, forensic and food safety applications, this resource also outlines workflow and selection processes to increase efficiency and assist with decision making.
SP Scientific
Authored by Dr. Margit Gieseler of GILYOS and Dr. Henning Gieseler of the Freeze Drying Focus Group at the University of Erlangen, this new technical article discusses how product resistance (Rp) has been recently suggested as a critical product parameter (CPtP) in freeze drying because it instantaneously draws a picture of the (already dried) inner cake morphology at the point of measurement. According to SP Scientific, from a physical point of view, Rp of the dried product layer impacts the resistance to water vapor flow and thus indirectly determines the maximally allowable shelf temperature and primary drying time. In addition to providing an introduction to product resistance behavior, the article summarizes the results of trials investigating the product resistance of sucrose based on different experimental conditions and using the Auto-MTM feature of the SP Scientific SMART Freeze Dryer software. The authors conclude resistance of the product to vapor flow during primary drying is a critical product parameter that impacts several process parameters. Lack of information about product performance during primary drying can lead to sub-optimal product quality and/or problems during scale-up. The use of MTM allows an assessment of product resistance data.
More: www.spscientific.com
Avid Nano
The new technical note (1107-T4) describes how a very small molecule with a low molecular weight (caffeine, MW 194.2 g/mol) was accurately measured at 1.4% concentration in aqueous solution. The results using Avid Nano's W130i DLS demonstrate that the light scattering signal from the dissolved caffeine is sufficient to produce a good quality correlogram with an intercept >0.2 and highly reproducible data with low polydispersity. The mean radius was consistently reported at about 0.48nm and molecular weight estimate around 400Da indicating concentration dependent self association into dimers, a known characteristic of caffeine in aqueous solutions.
Oxford Gene Technology
This new white paper by OGT discusses the relative merits of DNA microarrays and next generation sequencing (NGS) for human genome analysis. It discusses the essential factors to consider when selecting the optimal platform for a specific study, along with issues such as the suitability of microarrays and sequencing for various applications, the relative costs associated with each technology and how to overcome the challenges posed by the analysis of large and complex datasets.
Fluidigm
Informational videos by customers are now on the Fluidigm Web site. The first customer web video from Dr. Paul Robson of the Genome Insitute of Singapore reports on how he and fellow scientists have generated significant single cell expression data for a detailed molecular understanding of mammalian development from fertilization to embryo implantation, a process known as the preimplantation period. The knowledge gained has a direct impact on clinical applications in the areas of regenerative medicine and assisted reproduction. Another video from Professor Mylene Yao of Stanford University reports on how her group's work using Fluidigm IFC technology to undertake single embryo gene expression has allowed them to gain a better understanding of early embryo development.
Integrated DNA Technologies
IDT now offers an educational newsletter that features regular sections such as "Your Research," where readers can learn about research applications and findings plus sections dedicated to explaining fundamental scientific principles, tips and ideas to streamline research, "Ask Alex" and a light hearted science-humor feature.
More: www.idtdna.com/decoded
INTEGRA
A new application note demonstrates how combining INTEGRA's MEDIAJET Petri dish filler with the DOSE IT peristaltic pump provides an optimized automated solution for the production of high quality blood agar. The note describes an automated protocol that minimizes the time that the blood is exposed to hot agar medium and thus reduces thermally induced lysis of erythrocytes. By preparing the blood agar using an INTEGRA MEDIACLAVE media preparator, the dispensing temperature can be set and maintained at low temperature. By combining the MEDIAJET Petri Dish filler together with a DOSE-IT peristaltic pump and a special tubing set for blood agar the experimenters are able to add blood to the agar immediately before pouring the plate thereby reducing blood cell lysis significantly.
More: www.integra-biosciences.com/pdf/liquid_handling/Blood_agar_preparation_V00.pdf
TTP LabTech
A new application note from TTP LabTech illustrates how the company's Acumen eX3 laser scanning imaging cytometer can be used to elucidate novel cellular pathways involved in the shedding of the epidermal growth factor receptor (EGFR). The note describes how the high-throughput imaging technology of the Acumen eX3 quantifies and monitors protein expression levels.
According to TTP LabTech, online image capture and analysis, as well as multiplexing capabilities, allows the Acumen eX3 platform to provide consistent and accurate data from complex biological systems. The shedding of HB-EGF is classically controlled by a disintegrin and metalloproteinase, ADAM17. The imaging technology of Acumen eX3 is used to determine the contributions of additional enzymatic pathways in this process. HT1080 cells, transfected with a modified, alkaline phosphatase linked, heparin binding EGFR (AP-HB-EGF) are examined by both the traditional enzymatic assay and immunofluorescence for receptor shedding in the presence and absence of inhibitors of ADAM17 and PKC isoforms. Experimental data suggest that important contributions are made by the PKC isoforms α & δ. Disruption of EGFR homeostasis is associated with a number of important cancer targets.
More: www.ttplabtech.com/products/acumen/acumen-bibliography.html
Avid Nano
A new report from Avid Nano demonstrates how its BladeCell disposable cuvette improves dynamic light scattering (DLS) sample preparation time by a factor of 8 compared to using traditional quartz cuvettes. The 5µl BladeCell cuvette is precision molded in black polycarbonate and requires no sample preparation beyond centrifuging to reduce the possibility of dust contamination that can affect DLS measurements. To fill, 5µl of sample is simply pipetted against the walls of the BladeCell liquid trap where it is retained by surface tension. The prepared cuvette is placed into the temperature controlled sample holder of the Avid Nano W130i, where it can be analyzed by DLS in seconds. After analysis the sample is recovered by dipping a pipette tip into the liquid trap and removing the contents. Disposal of the BladeCell eliminates the cleaning steps required with quartz cuvettes.
Fluidigm Europe
This new technical note describes experimentation on a normal human genomic DNA sample using a prototype 200k Digital Array Chip on a modified BioMark System.
Fluidigm's Digital Array chips provide a platform to perform digital PCR and have been used in sample quantitation, rare mutation detection, haplotyping, and copy number variation studies. The new 200k chip prototype is more powerful and enables an even more precise and accurate measurement of target copy number. Two forms of commercial Digital Array chips are currently available, which have configurations known as 12.765 and 48.770 (with 9,180 and 36,960 reaction chambers each).
This report shows a prototype 200,000 chamber version of the Fluidigm Digital Array chip and demonstrates that the experimental process is further simplified and the copy numbers of two single copy genes (RnaseP and RPP30) can be measured at a much greater accuracy. The BioMark system used for the 200k chip experiments was modified by the addition of a higher resolution CCD camera.
More: www.fluidigm.com/home/fluidigm/Posters/Poster_AMN_2011_200K_dPCR.pdf
Exeter Analytical
This new paper discusses how analysis of the percentages of carbon, hydrogen and nitrogen in an organic sample (CHN microanalysis) can complement techniques such as mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectrometry to enhance reliability of analytical results. Data is provided showing how CHN analysis can help determine low levels of impurities, and how CHN microanalysis can be used to determine the presence of residual solvent in samples at levels that have little or no effect on the mass or NMR spectra.
Agilent Automation Solutions
This new guide shares information about how to optimize microplate sealing processes to safeguard the integrity of samples. It includes a microplate sealing tips section, information on how to optimize sealing parameters when thermal sealing, an FAQ section and an electronic PDF form designed to facilitate the capture of key information helpful when requesting technical/applications support.
More: www.chem.agilent.com/Library/datasheets/Public/5990-3483EN_LO.pdf
Agilent Automation Solutions
This new note demonstrates how the Bravo liquid handling system provides an efficient platform for automation of a SISCAPA Magnetic Bead Workflow for protein biomarker quantification by mass spectrometry (MS).
The authors describe an automated protocol implementing SISCAPA immunoaffinity enrichment of biomarker peptides prior to quantification by MS. In the described protocol, magnetic beads coated with anti-peptide antibodies are used to bind specific target peptides from tryptic digests of plasma and serum samples, after which the beads are washed to remove unbound peptides, and the bound, purified peptides are eluted in small volumes for injection and analysis in an LC/MS/MS system. Stable isotope labeled versions of the same peptides are used as internal standards to enable accurate quantification. The Agilent Bravo automated protocol for this application enabled SISCAPA processing of 96 samples in less than 30 minutes, less than half the time required by traditional manual methods.
More: www.chem.agilent.com/Library/applications/5990-7360en_lo%20CMS.pdf
Genevac
Genevac has launched a new web page with technological innovations, applications advances and references for evaporation and sample concentration techniques relating to food and beverage analysis. Web links are provided to a bibliography of scientific publications in peer review journals that offer further insight into how modern evaporative sample preparation methods are enabling laboratories to accelerate and improve the process of obtaining new compounds from a range of natural product sources.
More: www.genevac.com/food
Fluidigm
Using Fluidigm Corporation's BioMark System and Digital Array integrated fluidic circuits (IFCs), researchers from the University of California San Francisco (UCSF) have identified serum microRNAs that can serve as biomarkers for prostate cancer. The UCSF research group designed their own microRNA preAMP and PCR primers specifically to be used on the BioMark platform and then were able to identify mRNA signatures that correlate with prognosis.
To identify serum miRNAs that diagnose and correlate with prognosis of prostate cancer, the researchers at UCSF developed a multiplex quantitative reverse transcription PCR (qRT-PCR) method involving purification of multiplex PCR products followed by uniplex analysis on a Fluidigm microfluidics chip to evaluate 384 human miRNAs. Using Dgcr8 and Dicer knockout (small RNA - deficient) mouse ES cells (mESC) as the benchmark, the researchers confirmed the validity of their technique, while uncovering a significant lack of accuracy in previously published methods. Profiling 48 sera from healthy men and untreated prostate cancer patients with differing CAPRA (Cancer of the Prostate Risk Assessment) scores, UCSF researchers identified miRNA signatures that allowed them to diagnose cancer patients and correlate with prognosis. These serum signatures included oncogenic and tumor suppressive miRNAs suggesting functional roles in prostate cancer progression.
More: A copy of the UCSF paper "Serum microRNAs in Prostate Cancer" is available upon request from biomarkeurope@fluidigm.com. To review the human/mouse overlapping microRNA sequence and probe list designed by UCSF and validated on the Fluidigm BioMark System visit http://urology.ucsf.edu/blellochlab/protocols.htm.
Exeter Analytical
A filter-based technique, using an Exeter Analytical Model 440 CHN Elemental Analyser, provides rapid and routine analysis of marine sediments, results are expressed in micrograms of carbon and nitrogen detected simplifying the calculation process. Analytical sensitivity is less than 1 microgram.
Agilent Automation Solutions
The new white paper describes how the Agilent Bravo for Protein Purification Liquid Handling Platform together with AssayMAP high throughput microchromatography system was designed specifically to meet all of the technical requirements for quantitative sample preparation for protein analysis. The AssayMAP system is comprised of a microchromatography cartridge, special associated labware, and a liquid handling device designed to interface with the cartridge and provide precise flow control in a 96-channel microplate format.
The authors describe how applications of high-throughput microchromatography for protein analysis are designed to purify, quantitate and react. In purification, the goal is to separate the target protein from all or most of the impurities present in the sample, isolating the target for further operations. In quantitation, the goal is to measure the amount of the target protein present in the sample. In reaction, the goal is to modify the target protein chemically by either enzymes or other reactants. Sample preparation protocols may involve any or all of these types of steps.
TTP LabTech
A new comparative study demonstrates that the Mirroball antibody discovery system offers equivalent data to the discontinued ABI 8200 FMAT instrument, with minimal changes to assay protocols. The study uses the FMAT assay to look at bead-based quantitation of a soluble protein (rabbit IgG) and antibody binding to EGF receptors expressed in A459 cells. The Mirrorball system scanned the entire well, and FMAT reported data from only a 1 mm2 area in each well. Beads were identified by laser scatter on Mirrorball and fluorescence on FMAT. The Mirrorball system could enumerate beads in all wells, in contrast to FMAT, which could not detect all beads below 1 ng/mL rabbit IgG.
More: www.ttplabtech.com/emailers/mirrorball-benchmarking-study.html
Thermo Fisher Scientific
The first two videos uploaded to a new YouTube Chromatography Channel are entitled How GC Columns Work and An Introduction to SPE and offer training on how various aspects of chromatography work. The How GC Columns Work video tutorial uses an example application on the analysis of neutral and acidic drugs using Thermo Scientific TraceGOLD GC Columns to demonstrate how ultra low bleed, high levels of reproducibility and superior inertness are essential to ensure full confidence in analytical results. The Thermo Scientific Sample Preparation video demonstrates how, through the use of SPE, users can achieve a cleaner baseline and improve data.
More: www.youtube.com/chromatographyvideos
Porvair Sciences
The report details and illustrates the results from a series of comparative experiments carried out in an independent evaluation of a selection of protein precipitation microplates. The evaluation comments how the p3 microplate from Porvair Sciences eliminates wetting out and leaking of sample through the plate without resorting to complex valve systems. It also notes that the novel treated frit matrix used in the p3 maintains high flow rates enabling quick sample preparation times, and that the p3 plate did not have any major adverse non-specific binding problems.
More: www.porvair-sciences.com/downloads.php?showFrom=6&nR=18
Porvair Filtration Group
A report by Porvair Filtration Group describes how the open pore structure of its BioVyon sintered porous plastic materials offers optimum conditions for encouraging in-vitro cell culture and offers advantages over established Petri dish methods as well as the ceramic substrates (TCP) traditionally used. Results are presented for applications using BioVyon scaffolds to accelerate oesteoblast (bone cell) proliferation and provide efficient and accurate differentiation of adult and embryonic stem cells.
More: www.porvairfiltration.com/view/BiosciencesAndScientific/CellCulture.aspx
Integrated DNA Technologies (IDT)
This quantitative real-time polymerase chain reaction (qPCR) user guide provides guidance on the entire qPCR process - from RNA isolation to data analysis. The manual covers the basics of experimental set-up, performance and analysis. Specific information on 5' nuclease assays, including re-suspensions and qPCR protocols also are supplied, as well as a troubleshooting section. The entire document is written in compliance with MIQE guidelines: minimum information for publication of quantitative real-time PCR1.
More: www.idtdna.com
Fluidigm
Fluidigm's new website offers information relating to the company's proprietary technologies, product video demonstrations, a large database of application notes, articles and posters, customer success stories and a 2011 program of events.
More: www.fluidigm.com
Biotage
Seven new methods for the Biotage RapidTrace SPE Workstation are detailed in application notes, which Biotage users can receive preloaded with free software updates. Newly released methods include: Extraction of Vitamin D from Plasma Using ISOLUTE; Extraction of the Cocaine Metabolite, Benzoylecgonine from Urine using ISOLUTE HCX; Extraction of Tricyclic Antidepressants from Whole Blood Using ISOLUTE HCX Columns; Extraction of Basic Drugs of Abuse from Biological Fluids using ISOLUTE HCX; Extraction of Cyclosporine from Whole Blood Using ISOLUTE Columns; Extraction of Environmental Contaminants Including Dioxins and Furans from Soil and Sediment Samples Using ISOLUTE NH2; and Extraction of the Opiates, Morphine and Codeine from urine using ISOLUTE HCX.
More: www.biotage.com
Biotage
EtG can be detected at very low levels and being a metabolite it is useful in distinguishing between ingested ethanol and that which is absorbed through the skin from ethanol containing products such as alcohol based hand washes. Rapid and reliable methods with robust extraction protocols are essential for analysis and quantitation of EtG from various matrices. According to Biotage, advances in the manufacture of polymeric stationary phases and the recent release of EVOLUTE AX, a mixed-mode ion exchange product, allow development of a more robust, cost-effective procedure. The protocol is developed on 96-well plates for high-throughput with volumes outlined for scale-up to cartridges in manual or automated systems.
More: www.biotage.com
Agilent Automation Solutions
Integrating advanced automation and liquid handling with Agilent SureSelect XT workflow, Agilent Automation Solutions has created a fully optimized and validated high-throughput target enrichment solution. The solution, available in Agilent's online library, can result in throughput of up to 192 samples weekly per workstation.
Agilent Automation Solutions
A new technical note from Agilent Automation Solutions describes a BioCel System that offers the flexibility to accommodate different high-throughput screening (HTS) assays as well as new protocols and hardware. The system includes a selection of liquid handlers and readers, and an active environmental control for the entire system. A multimode reader, bulk reagent dispensers, plate washer and the Agilent Vertical Pipetting Station allow assays to be run in 96-, 384- or 1536-format. The active environmental control used by the Agilent BioCel System allows assays to be run at constant temperature (ambient up to 37°C), avoiding edge effects often caused by transport into and out of incubators. The high precision, low volume pipettor and the large plate storage capacity allow the compound plate bank to be run alongside the assay, adding the compounds on-the-fly. No prior assay plate generation is necessary and due to the low volume capacity of the Vertical Pipetting Station (200 nL), DMSO concentrations in the assay are kept low.
More: www.chem.agilent.com/Library/applications/5990-3619en_lo%20CMS.pdf
SP Scientific
Officially titled "Improving Lyophilization Manufacturing & Development with ContoLyo Nucleation On-Demand Technology (20100513 1302-1)," this new video by SP Scientific illustrates how Praxair's ControLyo Nucleation On-demand Technology (which is licensed by SP Scientific) can be used to control the nucleation of a product during freezing, such that the entire product nucleates at the same time and in the same way.
More: www.spscientific.com
