Tomasz Koprowski of the Institute for Chemical and Bioengineering in Zurich, Switzerland
The Tree of Life — C.elegans worms on a chunk of agar.
Ludovic Autin of The Scripps Research Institute, La Jolla, CA, USA
This image is a procedurally created subsection of synaptic vesicles.
Muhammad Awais of Royal Liverpool and Broadgreen University Hospitals, Liverpool, UK
Butterfly of depolarized and dead acinar cells -- Two clusters of primary cultures of acinar cells isolated from a mouse pancreas. Cells were loaded with JC-1 dye and the image was taken after the induction of depolarization of mitochondria. The imaging was done at the NIHR Liverpool Pancreas Biomedical Research Unit in UK.
Rakesh Karmacharya of Harvard University, Cambridge, MA, USA
Emergence of neurons from a bed of neural progenitor cells. The neural progenitor cells were derived from human induced pluripotent stem cells and cultured under conditions favorable to neuronal differentiations. The neural progenitor cells give rise to different neuronal and glial cells. Cells in red are neural progenitor cells (labeled with Nestin) and the ones in green are neurons (labeled with beta-III tubulin). Nuclei are in blue.
Josiane Lafleur of University of Copenhagen, Denmark
This microfluidic chip was designed for the monitoring of pollutants in the environment. The ultimate purpose of the chip transpired in its design, resulting in a flower-shaped meandering mixing channel.
Yi Lu of University of Arizona, Tucson, USA
Cell-ebrate Christmas — A Christmas tree shaped microchannel for rapid, high-throughput separation of endothelial spheroids from individual cells. The image captures endothelial cells and spheroids entrained with the fluid flows from top to bottom. The channel geometry creates two stagnation regions at the bottom of the channel for trapping spheroids in a size-dependent manner: small cells flow through the channel and large spheroids are trapped. The Christmas tree channel is fabricated by a rapid prototyping process based on a computer numerical control system with micrometer precision. The smallest feature that can be made by the system is 2 micron.
Alyce Nehme of University of New South Wales, Australia
A cluster of childhood neuroblastoma cells (SHEP) with their actin cytoskeleton stained with Phalloidin using a Leica SP5 two photon confocal microscope by merging several z slices.
Leo Price of OcellO, The Netherlands
Confocal image of a renal cyst cultured in a 3D extracellular matrix protein gel environment. Green: anti-ezrinimmunostaining; Blue: anti-epac1 immunostaining; Red F-actin staining. Environmental cues drive cell polarization and differentiation resulting in cyst formation - a structure which cannot be obtained in conventional 2D culture, but is central in the development of the disease. In collaboration with Leiden University, OcellO performs high-throughput screening of 3D cultured cysts derived from PKD1-depleted kidney epithelial cells, with the aim of identifying compounds to treat Polycystic Kidney Disease.
Constantinos Zeinalipour of Cardiff University, UK
This optical microscope picture depicts an array of micro-needles that can be used to deliver medicine to the human body painlessly. Such microchannels can also be used to extract skin fluids for the purpose of sensing or sampling. The micro-needles make micrometer holes to deliver a wider variety of active substances directly to the body. They are short enough to avoid capillaries and nerve endings that are located in the dermis making them less invasive.