High-Content Screening: An Introduction to Instrumentation, Assay Development, Screening, Image and Data Analysis – NEW for 2021

• Individuals who plan to develop or apply high-content analysis (HCA) / screening technologies
• Individuals who look to implement HCA/HCS infrastructure and capability at their organization or labs
• Individuals with first experience in HCA/HCS but want to get a systematic overview, learn more about good practice in assay development and how to manage and analyze large scale data

The course will provide:

• An introduction to all aspects of HCS including data analysis
• An overview on major categories and thereof examples of HCS instrumentation
• Guidance to assay selection and systematic optimization
• A demonstration of good practice in assay validation and screening
• An introduction to principles of image analysis and review of various commercial and open source software such as CellProfiler
• An introduction and examples of multi-parametric data analysis using supervised and unsupervised approaches
• Review of data analysis and visualization tools
• Presentation of case studies of assay development and screens
• On-demand discussion on hit verification and target deconvolution strategies

• Introduction to high-content analysis and screening (HCA / HCS)
• HCS instrumentation overview
• Reagents and assay development for HCS
• Image analysis
• Data management and analysis
• Case studies of high-content RNAi and small molecule screens

• Eberhard Krausz — ChemBioCon
• Steffen Jaensch — Janssen R&D (Pharmaceutical Companies of Johnson & Johnson)